Based on Marvel Comics' superhero character, this is a story of Peter Parker who is a nerdy high-schooler. He was orphaned as a child, bullied by jocks, and can't confess his crush for his stunning neighborhood girl Mary Jane Watson. To say his life is "miserable" is an understatement. But one day while on an excursion to a laboratory a runaway radioactive spider bites him... and his life changes in a way no one could have imagined. Peter acquires a muscle-bound physique, clear vision, ability to cling to surfaces and crawl over walls, shooting webs from his wrist ... but the fun isn't going to last. An eccentric millionaire Norman Osborn administers a performance enhancing drug on himself and his maniacal alter ego Green Goblin emerges. Now Peter Parker has to become Spider-Man and take Green Goblin to the task... or else Goblin will kill him. They come face to face and the war begins in which only one of them will survive at the end.
Peter Parker is an unhappy man: after two years of fighting crime as Spider-Man, his life has begun to fall apart. The girl he loves is engaged to someone else, his grades are slipping, he cannot keep any of his jobs, and on top of it, the newspaper Daily Bugle is attacking him viciously, claiming that Spider-Man is a criminal. He reaches the breaking point and gives up the crime fighter's life, once and for all. But after a failed fusion experiment, eccentric and obsessive scientist Dr. Otto Octavius is transformed into super villain Doctor Octopus, Doc Ock for short, having four long tentacles as extra hands. Peter guesses it might just be time for Spider-Man to return, but would he act upon it?
Peter Parker is finally happy: he is doing well at the school, his girlfriend MJ Watson loves him and the whole city loves his crime-fighting alter-ego Spider-Man, finally bestowing upon him the praise he so rightly deserves. Apart from this, he has made some new foes and rivals . His former friend-turned-foe Harry Osborn has become the New Goblin and wants to avenge his father by killing Spider-Man. On one night, an alien symbiote from outer space crash lands in Peter's vicinity and attaches itself to his Spider suit, turning it black in colour and enhancing its powers. Flint Marko, a fugitive, is turned into The Sandman when he gets subjected to a molecular lab experiment. Incidentally, Marko is the person who is revealed to have killed Peter's uncle Ben and now Peter wants revenge. Peter's job as a freelance photographer with The Daily Bugle is taken by Eddie Brock. Peter also learns that apart from enhancing his powers the black symbiote has also brought out the dark side within him which causes him to ruthlessly manages to throw Eddie out of his job. When MJ breaks up with him, he starts dating his fellow student Gwen Stacy who was Edie's ex, to make them both jealous. Soon Peter must decide whether he should let his dark side to dominate his personality or not.